Core Facilities

In situ Hybridization Core

Cecilia Ljungberg, Ph.D.

Contact Information

Email: cecilial@bcm.edu
Phone: 832-824-8873
Open 8 am to 5 pm Monday through Friday.

 Jan and Dan Duncan Neurological Research Institute,
1250 Moursund Avenue,
Office- N1365.02
Lab- N1350.25
Houston, TX 77030

The In Situ Hybridization Core performs non-radioactive RNA (mRNA or miRNA) In situ hybridization (ISH) on individual tissue sections and for high-throughput applications. A unique high-throughput technology developed by the Core is used to determine gene expression patterns with an emphasis on tissues from rodent experimental models.

Core services
The Core performs the following experimental steps of in situ hybridization -

Collecting animal tissue specimens
Preparation of frozen and paraffin sections
Preparation of RNA probes from customer templates
Conducting high-throughput ISH
X-gal and Cresyl Violet staining
Documentation and quantification of expression patterns by microscopy.
 

For human studies, customers must provide tissue sections. The Core assists users with dissecting and freezing tissue (fresh or fixed) and cryostat sectioning (sections on slides or free-floating sections).

For technical, scheduling or pricing questions, please contact the Core Director.