In situ Hybridization Core
The In Situ Hybridization Core performs non-radioactive RNA (mRNA or miRNA) In situ hybridization (ISH) on individual tissue sections and for high-throughput applications. A unique high-throughput technology developed by the Core is used to determine gene expression patterns with an emphasis on tissues from rodent experimental models.
The Core performs the following experimental steps of in situ hybridization:
- Collecting animal tissue specimens
- Preparation of frozen and paraffin sections
- Preparation of RNA probes from customer templates
- Conducting high-throughput ISH
- X-gal and Cresyl Violet staining
- Documentation and quantification of expression patterns by microscopy
For human studies, customers must provide tissue sections. The Core assists users with dissecting and freezing tissue (fresh or fixed) and cryostat sectioning (sections on slides or free-floating sections).
For technical, scheduling or pricing questions, please contact the Core Director.